Influence of Seasonal Variation and particle size on Quantitative Determination of Bio-active Markers in pluricaulis sp

Convolvulus pluricaulis (CP) is an indigenous perennial creeper herb in India used as a tonic in CharakSamhita that seems like morning glory. It showsvery important place in the Indian as well as Chineseherbaldrugindustrybecause, CP is one of the major ingredients in the marketed (>40)preparationsused for enhancingmemory and to treatvarious CNS disorders. The presence of coumarin component in CP i.e.scopoletin and scopolin has been foundto beresponsible for memoryenhancingactivity due to theiracetylcholinesteraseinhibitory action. The variation of seasonaffects the production of phyto-constituents and metabolites more thanthe otherfactors. The synthesis of chemicalmoietiesalso variesin accordance withoptimal conditions requiredatdiffernt duration of time period.In literature survey, pluricaulis shows maximum effect if it is collected in the month from April to August i.e. during its flowering season. Therefore, in order to find the scientific reason for collecting the plant in this season, CP was collected in two different seasons and markersquantity was determined. The modern powerful analytical technique called as planer chromatography, having separation power, performance and reproducibility superior to classic TLC method was utilized for quantitative determination of scopolin and scopoletin. Further, varient stress conditions can be investigated for both of the bio-active markers.